Journal: Nanoscale Advances

Article Title: Drug delivery with a pH-sensitive star-like dextran-graft polyacrylamide copolymer

doi: 10.1039/d2na00353h

Figure Lengend Snippet: Viability of the normal human lung fibroblast HEL299 cells, treated for 48 h with: (a) – free Dox or D- g -PAAan-Dox in Dox-equivalent concentrations, (b) – free Cis or D- g -PAAan-Cis in Cis-equivalent concentrations; * p < 0.05 compared to free drugs.

Article Snippet: Lung carcinoma human A549 and normal human lung fibroblast HEL299 cell lines were purchased from AddexBio Technologies (San Diego, CA, USA).

Techniques:

Journal: Nanoscale Advances

Article Title: Drug delivery with a pH-sensitive star-like dextran-graft polyacrylamide copolymer

doi: 10.1039/d2na00353h

Figure Lengend Snippet: IC 50 of free drugs and drug-loaded D- g -PAAan nanoparticles on HEL299 cell viability

Article Snippet: Lung carcinoma human A549 and normal human lung fibroblast HEL299 cell lines were purchased from AddexBio Technologies (San Diego, CA, USA).

Techniques:

Journal: Inflammation

Article Title: The Priming Potential of Interferon Lambda-1 for Antiviral Defense in the Oral Mucosa

doi: 10.1007/s10753-022-01624-1

Figure Lengend Snippet: Type III IFN receptor expression in human gingival keratinocytes. A : Detection of IL10R2 , IFNLR1 , and GAPDH mRNA in various keratinocytes and fibroblasts with (+) or without (-) cDNA by RT-PCR. M, marker. B : Quantitative analysis of IFNLR1 mRNA expression by real-time PCR in various keratinocytes and fibroblasts. Data represent the mean ± standard deviation (SD) of triplicate assays. C : Detection of cell surface IFN-λR1 by flow cytometry. The black line represents IFN-λR1, and the gray area shows the isotype control. A representative histogram from three independent experiments is shown. Values are the mean fluorescence intensity of IFN-λR1 expression and shown as a fold increase in IFN-λR1 expression from that with the control. D : HGK were treated with the indicated concentrations of IFN-λ1 for 30 min. Whole-cell lysates prepared from these cells were immunoblotted with anti-phospho-STAT1 (pSTAT1), anti-STAT1, or anti-GAPDH antibodies. A representative blot is shown. The bar graph shows the integrated signal intensities of the pSTAT1/STAT1 ratio. Data represent the mean ± SD of triplicate assays. * P < 0.05 and ** P < 0.01 versus untreated cells (0) (Dunnett’s multiple comparison test). E : Human gingival sections were stained with an isotype and anti-IFN-λR1 antibody. Sections were counterstained with hematoxylin. Representative images of 3 samples each are shown. The right image is a high-magnification image of the square area. A-D : Data show a representative of at least three independent experiments. OBA-9; an immortalized human gingival keratinocyte cell line. HGK; human primary gingival keratinocytes. HaCaT; an immortalized human skin keratinocyte cell line. HEK; human primary epidermal keratinocytes. HGF; human primary gingival fibroblasts. HFL-III; normal human lung fibroblasts.

Article Snippet: Normal human lung fibroblasts were provided by the Japanese Collection of Research Bioresources Cell Bank.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Marker, Real-time Polymerase Chain Reaction, Standard Deviation, Flow Cytometry, Control, Fluorescence, Comparison, Staining

Journal: BMC Molecular and Cell Biology

Article Title: Intracellular Ca 2+ is not essential for SHH signaling but is promoted by Shh ligand in embryonic fibroblasts

doi: 10.1186/s12860-026-00567-x

Figure Lengend Snippet: Quenching intracellular Ca 2+ does not affect SHH signaling transduction, but high concentrations of extracellular Ca 2+ do. A-C. Response of SHH signaling to manipulations of intracellular and extracellular Ca 2+ . NIH/3T3 cells were stimulated with Shh ( A ), ShhN ( B ), or 100 nM SAG ( C ) for 24 h in the presence of various concentrations of BAPTA or Ca 2+ , and Gli1 protein levels served as a readout for SHH pathway activity. The original Western Blot is provided in Supplementary Fig. . The data were representative of three independent experiments. Statistical analysis: Student’s t-test: ** P < 0.01. D. ATP levels in NIH/3T3 cells treated with SAG and/or Ca 2+

Article Snippet: NIH/3T3 cells (Keygen Biotech, China) were cultured to activate the SHH signaling pathway as described [ ].

Techniques: Transduction, Activity Assay, Western Blot

Journal: BMC Molecular and Cell Biology

Article Title: Intracellular Ca 2+ is not essential for SHH signaling but is promoted by Shh ligand in embryonic fibroblasts

doi: 10.1186/s12860-026-00567-x

Figure Lengend Snippet: Shh ligand promotes Ca 2+ influx after quenching intracellular Ca 2+ with BAPTA. A-B. The changes of intracellular Ca 2+ levels in NIH/3T3 cells treated with Shh conditioned media and/or BAPTA in the SOCE model: imaging (A) and quantification (B) of the changes of intracellular Ca 2+ levels. C-D. Similar to A-B, NIH/3T3 cells were treated with Shh conditioned media and/or Ca 2+ in the SOCE model: imaging (C) and quantification (D) of the changes of intracellular Ca 2+ levels. Statistical analysis: two-way ANOVA

Article Snippet: NIH/3T3 cells (Keygen Biotech, China) were cultured to activate the SHH signaling pathway as described [ ].

Techniques: Imaging

Journal: BMC Molecular and Cell Biology

Article Title: Intracellular Ca 2+ is not essential for SHH signaling but is promoted by Shh ligand in embryonic fibroblasts

doi: 10.1186/s12860-026-00567-x

Figure Lengend Snippet: Smoothened agonist SAG promotes Ca 2+ -induced calcium influx under basal conditions or in the presence of BAPTA. A-B. The changes of intracellular Ca 2+ levels in NIH/3T3 cells treated with SAG in the SOCE model: imaging ( A ) and quantification ( B ) of the changes of intracellular Ca 2+ levels. Statistical analysis: Student’s t -test: * P < 0.05. C-D. Similar to A-B, NIH/3T3 cells were treated with SAG and BAPTA in the SOCE model: imaging ( C ) and quantification ( D ) of the changes of intracellular Ca 2+ levels. Statistical analysis: two-way ANOVA. E-F. Similar to A-B, NIH/3T3 cells were treated with SAG and/or Extra Ca 2+ : Imaging ( E ) and quantification ( F ) of the changes of intracellular Ca 2+ levels in SOCE. Statistical analysis: two-way ANOVA

Article Snippet: NIH/3T3 cells (Keygen Biotech, China) were cultured to activate the SHH signaling pathway as described [ ].

Techniques: Imaging